BIO2071 ST: Biotechnology Lab Techniques

This hands-on laboratory course introduces students to molecular biology methods essential for analyzing genes and their products. Techniques covered include Gel Electrophoresis for separating DNA fragments based on size, Enzyme-Linked Immunosorbent Assay (ELISA) for quantifying specific molecules, Polymerase Chain Reaction (PCR) for amplifying specific DNA sequences, DNA subcloning for inserting genes into plasmid vectors, DNA purification for isolating high-quality genetic material, and DNA sequence analysis for determining the nucleotide order in a given DNA segment. As time permits, the course may delve into Western Blotting for detecting specific proteins in a sample, and Fluorescence In Situ Hybridization (FISH) for visualizing specific DNA sequences within cells. Emphasis is placed on proper lab techniques, maintaining lab safety, maintaining a welldocumented lab notebook, and utilizing quantitative methods for data analysis. Through a combination of theoretical understanding and hands-on practice, participants will develop a comprehensive skill set in molecular biology methodologies. Credits: 3.000

BIO1522 Lecture Min Credits: 4.00 $35.00